Extraction solvent used for the stems | Extraction yield (calculated from dry plant material) as w/w % | Elastase assay (% Inhibition ± SD) | Collagenase assay (% Inhibition ± SD) |
---|---|---|---|
Acetone | 3.36 | ** 91.25 ± 0.11 | 91.17 ± 3.59 |
Methanol: DCM (1:1) | 5.39 | ** 89.74 ± 0.32 | 93.75 ± 3.32 |
Ethanol | 3.09 | ** 93.35 ± 0.89 | 99.08 ± 3.57 |
Sequential extraction with hexane | 0.38 | ** 0 | ** 11.14 ± 2.51 |
Sequential extraction with DCM | 0.22 | ** 6.69 ± 1.36 | ** 19.62 ± 2.78 |
Sequential extraction with ethyl acetate | 0.87 | ** 65.02 ± 1.57 | 95.56 ± 1.68 |
Sequential extraction with methanol | 8.01 | 99.19 ± 0.18 | 94.46 ± 2.31 |
 |  | Controls |  |
10% MeOH |  | 3.28 ± 0.34 | 0 |
10% DMSO |  | 4.70 ± 0.53 | 1.58 ± 0.60 |
Elafin |  | 99.21 ± 0.05 |  |
EDTA |  |  | 95.66 ± 2.11 |