Fig. 4From: The benefit of combining curcumin, bromelain and harpagophytum to reduce inflammation in osteoarthritic synovial cellsLPS induces gene expression and release in medium of catabolism, inflammation and pain markers. Human synovial cells were treated with LPS (1 μg/ml) for 24 h. A - At the end of the experiments, RNA was extracted. Relative mRNA expression of MMP-1, MMP-3, MMP-13, NGF and IL-6 was determined by RT-PCR. Values are compared to untreated cells and presented as a log Fold Change (compared to the control group). B - Culture media were collected and ELISA was performed to assayed MMP, IL-6 and PGE2 concentration in medium. Values are expressed as μg/ml medium (n = 4). *: p-value< 0.05, **: p-value< 0.01, ***: p-value< 0.001Back to article page