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Fig. 2 | BMC Complementary Medicine and Therapies

Fig. 2

From: Rhein promotes the proliferation of keratinocytes by targeting oestrogen receptors for skin ulcer treatment

Fig. 2

Effects of Rhein on the viability and proliferation of keratinocytes. A Structure of rhein. B Cell viability of keratinocytes was tested using the CCK-8 assay. C Rhein-treated HaCaT cells was labeled with BrdU, and measured by flow cytometry and staining with anti-BrdU antibody. Region 3 (R3) is G0/G1, region 4 (R4) is S, and region 5 (R5) is G2/M. The representative scatter diagrams of each group (DMSO group, Rhein 25 μM group and Rhein 50 μM group) are shown. D The results of cell cycle were analyzed by Flowjo version 10.0.7 software. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001 vs DMSO group; #P < 0.05, ##P < 0.01, and ###P < 0.001 vs the 25 μM rhein group

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