Compounds | Concentration (ng/mL) | matrix effects (%) | Recovery (%) | Precision (RSD%) | Accuracy (%) | Room temperature 24 h | Three freeze- thaw cycles | In 56 ℃ for 1 h | ||
---|---|---|---|---|---|---|---|---|---|---|
Intra-day | Inter-day | Intra-day | Inter-day | measured value | measured value | measured value | ||||
100 | 96.55 ± 2.09 | 95.92 ± 2.07 | 5.85 ± 5.78 | 5.19 ± 5.18 | 98.73 ± 5.78 | 99.76 ± 5.18 | 99.68 ± 2.31 | 97.89 ± 4.06 | 98.46 ± 2.77 | |
AST | 200 | 102.99 ± 2.75 | 92.76 ± 2.47 | 2.85 ± 2.71 | 3.98 ± 3.87 | 95.05 ± 2.71 | 97.36 ± 3.87 | 199.01 ± 3.99 | 193.37 ± 7.61 | 194.85 ± 6.53 |
500 | 100.24 ± 2.28 | 94.96 ± 2.16 | 2.86 ± 2.82 | 2.43 ± 2.43 | 98.93 ± 2.82 | 99.96 ± 2.43 | 498.07 ± 3.95 | 497.15 ± 6.61 | 495.21 ± 8.43 | |
50 | 107.61 ± 2.45 | 89.83 ± 2.04 | 7.89 ± 8.08 | 6.33 ± 6.35 | 102.43 ± 8.08 | 100.4 ± 6.35 | 49.14 ± 2.35 | 49.41 ± 1.85 | 48.33 ± 2.76 | |
FE | 125 | 93.48 ± 4.51 | 90.13 ± 4.35 | 2.18 ± 2.19 | 2.61 ± 2.63 | 100.37 ± 2.19 | 100.68 ± 2.63 | 122.72 ± 2.65 | 124.36 ± 2.46 | 123.42 ± 1.76 |
400 | 88.56 ± 2.09 | 96.54 ± 2.74 | 1.45 ± 1.48 | 1.84 ± 1.88 | 102.23 ± 1.48 | 101.92 ± 1.88 | 403.59 ± 7.84 | 400.59 ± 6.95 | 399.13 ± 3.26 | |
20 | 92.25 ± 2.35 | 95.84 ± 2.44 | 8.48 ± 8.75 | 6.01 ± 6.25 | 103.23 ± 8.7 | 104 ± 6.25 | 20.81 ± 0.85 | 20.62 ± 1.06 | 19.88 ± 0.43 | |
PRO | 100 | 108.34 ± 1.53 | 90.61 ± 1.28 | 6.67 ± 6.62 | 4.78 ± 4.89 | 99.17 ± 6.62 | 102.15 ± 4.89 | 100.34 ± 1.48 | 97.77 ± 2.49 | 98.21 ± 2.33 |
400 | 105.24 ± 3.06 | 91.32 ± 2.65 | 4.3 ± 4.43 | 3.45 ± 3.48 | 103 ± 4.43 | 101.05 ± 3.48 | 398.27 ± 4.21 | 395.66 ± 7.14 | 394.26 ± 8.01 | |
100 | 103.81 ± 3.10 | 89.57 ± 2.67 | 4.51 ± 4.36 | 3.62 ± 3.56 | 96.56 ± 4.36 | 98.34 ± 3.56 | 99.91 ± 2.94 | 98.55 ± 2.93 | 97.22 ± 3.31 | |
CH | 200 | 102.10 ± 2.23 | 95.61 ± 3.02 | 4.96 ± 4.69 | 5.13 ± 4.96 | 94.62 ± 4.69 | 96.84 ± 4.96 | 200.03 ± 9.56 | 191.77 ± 8.91 | 195.56 ± 7.88 |
500 | 93.58 ± 1.13 | 97.97 ± 1.18 | 6.84 ± 6.75 | 4.57 ± 4.54 | 98.66 ± 6.75 | 99.58 ± 4.54 | 499.00 ± 6.02 | 485.90 ± 9.73 | 489.60 ± 8.91 | |
100 | 102.84 ± 2.17 | 90.62 ± 1.91 | 5.87 ± 5.68 | 4.09 ± 4.03 | 96.67 ± 5.68 | 98.7 ± 4.03 | 99.44 ± 2.43 | 99.68 ± 2.83 | 95.32 ± 1.06 | |
PE | 200 | 107.07 ± 0.71 | 98.69 ± 0.65 | 1.71 ± 1.75 | 2.53 ± 2.60 | 102.79 ± 1.75 | 102.68 ± 2.60 | 199.64 ± 2.59 | 197.55 ± 3.60 | 196.25 ± 4.08 |
500 | 100.02 ± 6.36 | 94.55 ± 6.01 | 1.83 ± 1.84 | 2.6 ± 2.62 | 100.82 ± 1.84 | 100.71 ± 2.62 | 499.23 ± 2.58 | 492.85 ± 7.60 | 495.87 ± 6.50 | |
200 | 91.94 ± 3.62 | 97.90 ± 3.85 | 3.83 ± 3.69 | 5.12 ± 5.10 | 96.23 ± 3.69 | 99.64 ± 5.10 | 208.88 ± 8.46 | 194.10 ± 8.56 | 196.22 ± 3.40 | |
SAB | 400 | 101.21 ± 1.16 | 92.44 ± 1.06 | 1.13 ± 1.03 | 3.2 ± 2.95 | 90.92 ± 1.03 | 92.09 ± 2.95 | 391.06 ± 6.16 | 388.99 ± 6.75 | 395.20 ± 10.70 |
800 | 102.70 ± 1.34 | 99.81 ± 1.30 | 3.57 ± 3.39 | 3.2 ± 2.97 | 94.98 ± 3.39 | 92.82 ± 2.97 | 792.27 ± 3.94 | 790.71 ± 3.52 | 791.11 ± 5.98 |