Fig. 2

Effects of TJ-41 on C2C12 myotube morphology. A Representative images of C2C12 myotubes (20×). Differentiated C2C12 myotubes were subjected to serum starvation for 48 h with vehicle (DMSO) or TJ-41 extract (10 or 100 µg/mL). For visualization of cytoskeleton (F-actin), cells were stained with fluorescently-labeled phalloidin. Red bar indicates 100 μm. B Box plot of C2C12 myotube width in each group. The number of fibers analyzed in each group is 174, 158, 168, and 106, respectively. Significant differences were detected by the post-hoc Tukey-Kramer test. *p < 0.05, ***p < 0.001