Fig. 6

The effect of CLE on the HO-1 expression (A), and Nrf2 nuclear translocation (B, C) in BV2 cells. Cells were treated with CLE for 12 h at indicated concentrations. The levels of HO-1, cytosolic Nrf2, and nucleic Nrf2 were determined by Western blot analysis. β-Actin and PCNA were used as a loading control for cytoplasm and nuclear, respectively. Representative blots from three independent experiments are shown