Fig. 4

Effect of AP and andrographolide on Nrf2, Keap1, p62 and LC3B-II protein expression in the hippocampus of alchor/D-gal induced AD mice. The expression levels of Nrf2, Keap1 (A), p62 and LC3B-II (D) protein were examined by western blot, and GAPDH was served as loading control; Quantitative analysis of Nrf2 (B), Keap1 (C), p62(E) and LC3B-II (F) protein expression. Values are mean ± SD (n = 6–8). ^*P < 0.05, ^**P < 0.01 versus the normal, ^#P < 0.05, ^##P < 0.01 versus model group