Fig. 5

Effect of AP and andrographolide on cell viability and LC3 proteins expression in the AD cell model. (A)PC12 Cells were treated with a range of AP concentrations (0.94,1.88,3.75,7.50,15.00 and 30.00 µg/mL) for 24 h; (B)Cells were co-treated with 700 µM Al(mal)₃ and various concentrations of AP (1.25,2.50,5.00,7.50.10.00 and 15.00 µg/mL) or 10 µM Andro for 24 h. Cell viability was measured by MTT assay. (C)After transfection with ptfLC3, PC12 cells were incubated with 700 µM Al(mal)₃ and 5.00 µg /mL AP or 10 µM Andro for 24 h. The images of LC3 protein expression were recorded by a fluorescence microscope. (D, E)The number of autophagosome (yellow) and autophagolysosome (red) dots per cell were quantified. Values are mean ± SD (n = 3). *P < 0.05, **P < 0.01 ,***P < 0.001versus the normal, ^#P < 0.05, ^##P < 0.01 versus Al(mal)₃ group