Fig. 6
Effect of siRNA p62 on Nrf2, Keap1, p62 and Tau mRNA and proteins expression in the AP-treated AD cell model. After transfection with p62 siRNA, PC12 cells were incubated with 700 µM Al(mal)3 and 5.00 µg /mL AP for 24 h. The levels of p62(A), Nrf2(B) and Keap1 (C) mRNA were analysed using RT-qPCR. (D) The expression of p62, keap1, Nrf2 and Tau were measured by western blotting, and GAPDH was served as a loading control. Quantitative analysis of p62 (E), Keap1 (F), Nrf2(G) and Tau (H) protein expression. Values are mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001